Working and Application of High Performance Liquid Chromatography (HPLC)
Keywords:
High Performance Liquid Chromatography, Working and Application of (HPLC)Abstract
Chromatography has existed for a long time and is still used to separate compounds. Analyzing a broad variety of application areas is made possible by high-performance liquid chromatography (HPLC). In this article, we will go over the basics of high-performance liquid chromatography (HPLC), including how it works, what parts make up an HPLC system, and what variables impact the accuracy of a test. This new advanced HPLC category uses the same fundamental theory and methods as its predecessors but boasts superior chromatographic performance. This paper aims to provide a comprehensive overview of UPLC, including its principles, instruments, and applications.
References
Ali, I., Alam, S. D., Raja, R., Khan, S. A., Anjum, R., Jain, A. K., Locatelli, M. (2023). Advances in Simple and Chiral-HPLC Methods for Antiallergic Drugs and Chiral Recognition
Ali, I., Alam, S.D., Raja, R., Shirsath, V., Jain, A.K., Yusuf, K., Aljuwayid, A.M., Sillanpää, M. (2022). Chiral separation of β-blockers by supercritical fluid chromatography using Chiralpak-IG and Chiralpak IBN-5 columns. Chirality, 34(6), 848-855.
Ali, I., Raja, R., Alam, S.D., Shirsath, V., Jain, A.K., Locatelli, M. David, V. (2021). A comparison of chiral separations by supercritical fluid chromatography and high-performance liquid chromatography. Journal of Liquid Chromatography & Related Technologies, 44, (11-12), 450-463.
Al‐Othman, Z. A., Al‐Warthan, A., Alam, S. D., & Farooqi, J. A. (2014). Enantiomeric separation and simulation studies of pheniramine, Oxybutynin, Cetirizine, and brinzolamide chiral drugs on amylose-based columns, Chirality, 26(3), 136–143.
Asif, M., Yusuf, M., Almehmadi, Alsaiari, AA, Allahyani, M., Aljuaid M. and Alsharif, A (2024). Towards Antiviral Potential of Biomolecules Derived from Adhatod avasica as Competent Natural Molecules to Treat COVID-19 Virus Variant. Letters in Organic Chemistry, 21(5), 466-477.
da Costa, L. M., Oliveira de Almeida Leite, H., Kassab, N. M., & Singh, A. K. (2019). Green analytical methods for the separation of seven antihistamines: Application in separation of azelastine and related impurities in nasal solution. International Journal of Analytical Chemistry, 2019(9489723), 1-11.
Mechanism. Analytica, 4(1), 66-83.).
Tao, Q.F., Zhu, X.P. and Zhao, Y. (2007). Chiral resolution of cetirizine by capillary electrochromatography using no vancomycin hydrochloride as chiral selector,” Chinese Journal of Pharmaceutical Analysis, 27(5), pp. 686–688.
Xiong, X., Wang, K., Tang, T., Fang, J., & Chen, Y. (2021). Development of a chiral HPLC method for the separation and quantification of hydroxychloroquine enantiomers, Scientific Reports, 11(1), 1-18.
Yusuf, M. (2023). Insights into the in-silico research: Current scenario, advantages, limits, and future perspectives. Life In Silico, 1(1), 13-25. https://life-insilico.com/index.php/pub/article/view/5 10.
Zhou, J., Luo, P., Chen, S., Meng, L., Sun, C., Du, Q., & Sun, F. (2016). Enantioseparation of six antihistamines with immobilized cellulose chiral stationary phase by HPLC. Journal of chromatographic science, 54(4), 531-535.
